Abstract: In present study, the variation in the concentrations of inorganic As species (penta and trivalent) in ground water of coal mining area (2nd aquifer) and its drain outlet/discharge in a reservoir/gorano dam. The water samples were collected from reservoir at four different sites (RW1, RW2, RW3 and RW4. At site four of reservoir (RW4) the fish farming is also carried out, from where a fish species Tilapia (Oreochromis mossambicus) was also collected. The solid phase extraction using titanium dioxide as an adsorbent was used for the determination of inorganic As. The arsenite (As^III) was found by an advance extraction method (cloud point extraction) using a hydrophobic chelating reagent. Physico-chemical, parameters of all water samples collected from ground water of aquifer 2 of coal mining area (AQ2), drain/discharge outlet (DW) and four sites of reservoir/Gorano dam were carried out. The resulted data indicated that physicochemical parameters (conductivity, total dissolved salt, pH) of all collected samples were surpassed the WHO recommended values for drinking water excluding pH. The different organs (gills, scale, liver and muscles) of O. mossambicus were analyzed for total As. The resulted data designated that accretion of As in various tissues of selected fish species was found in decreasing order as liver>gill>muscles >scale. The bioaccumulation factor was calculated >28. To evaluate the exposure of As via consuming the fish muscles by people was calculated by daily intake of As (EDI), and found to be significantly higher than the allowable limit (2.1 µg/Kg/day) by food organizations.

Biography: Professor T.G. Kazi did her Masters in Biochemistry in 1972 from University of Sindh, securing second class First position. She obtained Ph.D. degree in Bioanalytical Chemistry in 1978 from Centre of Excellence in analytical Chemistry, and worked in same institute as postdoctoral research fellow for two years (1979-80). She was appointed as Assistant Professor in January 1981 in the same Centre and promoted through ranks to full Professor in May 1996. During 1986 Dr. Kazi was offered postdoctoral fellowship under US-AID for research at state University of Rutgers, Camden USA.

Dr Kazi is a Leading researcher and versatile educator with distinguished academic carrier producing 40 Ph.D., 25 M.Phil. and 7 MS. research scholars. She also supervise many research scholars of biological science, Medical universities, Biochemistry, Botany and Fresh water biology. Honored with International and National Awards：
• Top cited professor among 2% scientist all over the world and among 81 scientists in Pakistan;
• Top analytical scientist among 60 scientists in all over the world and 10 of Asian country (Dr Kazi is only Muslim scholar selected up till now);
• International publications: 400+
• Impact factor > 1000
• Citation 11000.
• Azaz-e- Fazeelat, by President of Islamic Republic of Pakistan

About 47 years academic and Research experience (1973-2020) in Chemistry laboratories of National Centre of Excellence in Analytical chemistry, she has well acquainted with the state of art in modern instrumental techniques specialized in multidisciplinary research related to Environmental pollution including water, soil, waste water and sludge, quality control measurement for consumer commodities, drugs, edible oils, food, soft drinks, mineral bottled water and spices and coal produce in different areas of Pakistan. Actively committed to the field of research yielding over Recently work on the arsenic contamination of ecosystems of Sindh and coal mining impact on environment and published number of international papers with high impact factor, on its deterioration and impact on human health.